• If you are citizen of an European Union member nation, you may not use this service unless you are at least 16 years old.

  • Whenever you search in PBworks or on the Web, Dokkio Sidebar (from the makers of PBworks) will run the same search in your Drive, Dropbox, OneDrive, Gmail, Slack, and browsed web pages. Now you can find what you're looking for wherever it lives. Try Dokkio Sidebar for free.



Page history last edited by Eliana Tucker 1 year, 9 months ago

Methanol and acetic acid graphical abstract .pdf Methanol and acetic acid graphical abstract .pdf Methanol and acetic acid graphical abstract .pdf Methanol and acetic acid graphical abstract .pdf Methanol and acetic acid graphical abstract .pdf Methanol and acetic acid graphical abstract .pdf  Poplar esterified cell wall transformations and metabolic integration (PECTIN) study 








Kolby JardinePlant Physiology, biochemistry of volatile organic compoundsplant physiology, LBNL


Rebecca Dewhirst, biochemical ecology, LBNL

Cassandra Afseth, biomedical engineering, Illinois

Pubudu HandakumburaMulti-omics approaches in Molecular Plant Phenotyping, PNNL


Robert Young, NMR spectroscopy applications in biophysicalchemistry, PNNL


 Jenny Mortimer, Plant cell wall biosynthesis and metabolism, Plant Systems Biology, JBEI 


Nancy Washtongel-state and solid-state NMR chemical analysis of cell walls and metabolism, EMSL


 Mary S. Lipton, Carbon isotope methods in mass spectrometry and plant metabolism, EMSL


Cristina Castanhasenior research associate, LBNL


Eliana Tucker, student assistant, LBNL



Eoin Brodie, Deputy Directory of CESD, Environmental and Biological Systems Sciences and Microbes-to-Biomes, LBNL 


Henrik Vibe Scheller, Vice President for Feedstocks and Director of Cell Wall Biology and Engineering, JBEI


 Aymerick Eudes, Deputy Director of Cell Wall Biology and Engineering, JBEI


Nate McDowell, non-structural carbohydrates and plant response to drought PNNL/EMSL 


Wellington Muchero, Quantitative geneticist, Poplar genome wide associate study, ORNL


Alex Guenther, Atmosphere Integrated Research, UCI


Breeanna Urbanowicz, University of Georgia, UGA 












Polysaccharides are major components of plant cell walls that can be converted into fuels by microbial fermentation, making plant biomass an important bioenergy resource. However, a substantial fraction of plant cell wall polysaccharides are chemically modified with methyl and acetyl groups that can reduce fermentation yields. Although little is known about the biochemical and physiological functions of those cell wall modifications in trees, recent evidence in Arabidopsis suggests that they are critical for proper development and functioning of xylem vessels and leaf stomata. While generally considered a waste product of cell wall physiochemical changes, we recently demonstrated that plant-atmosphere emissions of the volatile intermediates methanol and acetic acid are tightly associated with tree growth, stress, and senescence processes and can be both emitted to the atmosphere via stomata and integrated into central C1 and C2 plant metabolism. However, methanol and acetic acid are not captured by traditional metabolomics analysis, representing an important gap in our knowledge of cell wall metabolism and its interactions with the environment. To address these knowledge gaps, a DOE BER early career research project (ECRP) was recently funded entitled, “O-Acetylation and methylation engineering of plant cell walls for enhanced biofuel production”. This 5-year project started Nov 1st, 2018 will study the metabolism of those cell wall modifications and volatile intermediates as well as their role in central physiological processes in the emerging biofuel tree species California poplar (Populus trichocarpa) using field settings and controlled environmental conditions.



PECTIN Publications

  1. Dewhirst R, Mortimer J, Jardine K (2020) Do cell wall esters facilitate forest response to climate?  Trends in Plant Science. https:/doi.org/10.1016/j.tplants.2020.05.011
  2. Dewhirst R, Afseth C, Castanha C, Mortimer J, and Jardine K (2020) Cell wall O-acetyl and methyl esterification patterns of leaves reflected in atmospheric emission signatures of acetic acid and methanol, PLoS ONE 15(5), e0227591. https://doi.org/10.1371/journal.pone.0227591 
  3. Dewhirst R, Handakumbura P, Clendinen C, Arm E, Wang W, Washton N, Young R, Mortimer J, McDowell N, Jardine K (2021) Temperature acclimation of leaf gas exchange and photochemistry in Populus trichocarpa, ACS Earth and Space Chemistry, 5, 8, 1813–1828.

  4. Dewhirst R, Lei J, Afseth C, Castanha C, Wistrom C, Mortimer J, Jardine K (2021) Are Methanol-Derived Foliar Methyl Acetate Emissions a Tracer of Acetate-Mediated Drought Survival in Plants? Plants, 10, 411.














Relevant books and papers (published)




Comments (0)

You don't have permission to comment on this page.